Adaptyv RFdiffusion IL-7Rα binder BLI revalidation

Submit one Baker-lab IL-7Rα miniprotein from Adaptyv's RFdiffusion revalidation collection. Guardians score kd fields on the hashed snapshot. They do not run BLI.

Adaptyv retested RFdiffusion-designed IL-7Rα miniproteins (Cao et al. / Baker lab designs) with a standardized BLI affinity workflow and listed sequences plus kinetic fields on Proteinbase collection rfdiffusion-re-validation. This is a methods retest table, not the EGFR or TREM2 community contests. Forty-two proteins are in the hashed snapshot; 15 have experimental kd fields. The author field is empty on these rows.

This bounty purchases a match to one row in that hashed snapshot. It does not purchase a new wet-lab campaign and it does not purchase authenticated laboratory provenance.

Definitions And Scope

A candidate is the amino acid string in binder.fasta. A match is the unique row with that sequence. Success means that FASTA string is present on exactly one hashed-snapshot row that also has the required expressed, binding, and kd fields defined below. It does not establish that those fields are authentic laboratory BLI measurements, a new physical sample, or that the Solver performed the Baker-lab design protocol.

Required Outputs And Format

binder.fasta: one > header, then amino acids. Ignore the header. Strip ASCII whitespace. Nonempty. Only ACDEFGHIKLMNPQRSTVWY.

methods.md must contain the matched row's name and designMethod as case-sensitive contiguous substrings. Do not require author; it is empty in this snapshot. It must contain this exact sentence, including the period:

No new laboratory BLI was performed for this Submission.

Package rules:

• archive format: none; one flat directory;
• only binder.fasta and methods.md.

Access And Known Limitations

Guardians fetch that URL themselves and check SHA-256 of the raw bytes, including a UTF-8 BOM if present, with no decode before hashing. evaluations JSON includes bli_kinetic_curves. Do not fetch curve URLs. Missing access or a hash mismatch blocks judgment. There is no Proteinbase signature, notarization, or signed manifest for this snapshot. Guardians cannot independently prove Proteinbase authored the bytes. This bounty purchases historical analysis of the exact bytes whose SHA-256 is listed above.

Parse the hashed bytes as follows. Decode as UTF-8-SIG (BOM stripped for parse only). Then read records with Python 3 csv.reader using the excel dialect and no other options: delimiter is the ASCII comma ,; quote character is "; doublequote is true so a literal " inside a quoted field is encoded as ""; skipinitialspace is false; quoting is csv.QUOTE_MINIMAL. Do not split on commas with a regex, semicolon, or tab.

The first record is the header row. Header cells are the cell text after CSV unquoting. Required header names, each exactly once and in this order: id, name, sequence, author, designMethod, evaluations. Extra columns after those six are ignored. Missing, reordered, or duplicate required headers: the snapshot cannot be used and judgment is blocked.

Every later record is one data row. Map cells to headers by name. If csv.reader raises csv.Error, or a record has fewer fields than the header, skip that record; it is not a data row and cannot match. Extra fields beyond the header are ignored.

The evaluations cell after CSV unquoting is a Unicode string. Decode it with Python 3 json.loads. If json.loads raises json.JSONDecodeError, TypeError, or ValueError, or the result is not a JSON array (Python list), that row has no evaluations objects.

• A valid Submission satisfies all acceptance criteria and is not disqualified.
• Lowest geometric-mean kd wins. Binary64 ties go to the lowercase Solver address that sorts first.
• If no Submission is valid, the outcome is no_valid_submission.

• required artifacts missing after successful retrieval and decryption;
• the decrypted directory contains any filename other than binder.fasta and methods.md.

UTF-8 decode failure, FASTA parse failure, or Markdown that cannot be read as UTF-8 text fails the corresponding Pass/Fail check. There is no separate “corrupt artifact” disqualification.

Guardians must not rank by esmfold_plddt or TM-score fields. methods.md may mention BLI, RFdiffusion, or computational scores; those mentions do not disqualify and do not change the score. Non-rfdiffusion rows fail Pass/Fail Check 5. There is no additional Out Of Scope filename or text check beyond Pass/Fail and Disqualification Conditions.

Allowed Resources And Reuse

Published retest designs listed in the hashed snapshot may be submitted. Disclose name and designMethod.

Judge only submitted artifacts, this page, and the listed CSV. Do not run BLI.

Evaluation Procedure And Limits

1. Fetch il7ra_revalidation.csv and check SHA-256.
2. Open binder.fasta and methods.md.
3. Apply Pass/Fail Checks.
4. Geometric-mean experimental kd.
5. Apply the winner rule.

One pass over 42 rows is enough.